Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie
Article Title: Artemisinin protects dopaminergic neurons against 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced neurotoxicity in a mouse model of Parkinson's disease.
doi: 10.1016/j.biopha.2023.115972
Figure Lengend Snippet: Fig. 6. Nrf2 inhibition neutralizes the protective effect of artemisinin following 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine (MPTP)-mediated intoxication. An Nrf2 inhibitor (ML385; 50 mg/kg, i.p.) or saline was injected once daily, 30 min before artemisinin treatment in the MPTP-mediated intoxication model. Dopa minergic neurons were visualized via tyrosine hydroxylase (TH)-specific immunostaining (A). The TH-immunopositive neurons in the substantia nigra pars compacta (SNpc) were counted (B) and the optical density in the striatum (ST) was measured (C). The pole test was conducted 7 days after the last MPTP injection (D, E). ##p < 0.01 and ###p < 0.001, compared with the control group; *p < 0.05, * *p < 0.01, and * **p < 0.001, compared with the MPTP-treated group.
Article Snippet: The following antibodies and ELISA kits were used in this study: anti- tyrosine hydroxylase (TH; Millipore Bioscience Research, MAB315 and AB152: Bedford, MA, USA), Anti-HO-1 (Abcam, ab204524: Cambridge, UK and Enzo Life Sciences, ADI-SPA-895D: Farmingdale, NY, USA), Anti-NQO1 (Santa Cruz Biotechnology, sc-32793: Heidelberg, Germany), TransAM assays for Nrf2 (Active Motif, 50796: Carlsbad, CA, USA), Dopamine High Sensitive ELISA (Rocky Mountain Diagnostics, #BA E-5300R: CO, USA), Rat tyrosine hydroxylase (TH) kits (Cusabio, CSB-E13102r: Houston, TX, USA), Rat dopamine transporter (DAT) kits (Elabscience, E-EL-R0343: Houston, TX, USA), MitoProbe JC-1 assay kits (ThermoFisher, M34152: Cleveland, OH, USA), and colorimetric cleaved caspase-3 (Clontech, 630217: Palo Alto, CA, USA).
Techniques: Inhibition, Saline, Injection, Immunostaining, Control